jenny-england 150x190
Jenny England
Staff Scientist, Innovation & Applications
Thermo Fisher

Optimization of an Anion Exchange Method for
the Large-Scale Production of Plasmid DNA for Gene Therapy and DNA Vaccine Applications

November 18, 2021, at 11 am ET (8 am PT | 5 pm CET)

In this webinar, we will discuss the optimization of a capture step for the purification of a 5.3 kbp pDNA using Thermo ScientificTM POROSTM AEX resins. The Design of Experiments (DoE) approach, using small high-throughput screening columns, was completed to determine the optimal conditions for three different anion exchange chromatography resins with different surface chemistries. The greatest dynamic binding capacity was observed for a weak ion exchange resin (15 g/L) on 1-mL columns and these optimized conditions were confirmed using a scaled-down model. The advantages of POROSTM resins in terms of pDNA binding capacity and impurity removal will be discussed to illustrate the capabilities of these chromatography media to facilitate the development of a large-scale plasmid purification process that can meet the growing demands of gene therapy and vaccine therapeutics.


Hana Jug
Project Manager in Process Development for Viral Vectors and Vaccines

Adenovirus Vector Manufacturing

Platform Using CIMmultus QA

November 30, 2021, at 11 am ET (8 am PT | 5 pm CET)

In this webinar, the speaker from BIA Separations, a Sartorius company, will share the advantages of using a platform for purification of Adenoviral vaccines using market leading CIM® monolithic chromatographic columns and an analytical toolbox for process monitoring in Adenoviral vaccine production.




Dr. Adil Mohammaed, Staff Fellow, US FDA (upper left);Dr. Chikkathur Madhavarao, Biologist, US FDA (upper right); and Robert Thomas, CSci, CChem, FRSC, Principal Consultant, Scientific Solutions

Practical Approaches to Metals Analysis of
Cell Culture Media & Impact on
Therapeutic Protein Production Using ICP-MS

December 2, 2021, at 11 am ET (8 am PT | 5 pm CET)

Metal ions can act as enzyme cofactors and can directly influence the kinetics of biochemical reactions that may also influence the biological production and quality attributes of therapeutic proteins. As a result, variations of the metal content in the cell culture media can affect the cell growth and production of the biologic product as well as its critical quality attributes (CQAs). The webcast will focus on the use of inductively coupled plasma mass spectrometry (ICP-MS) to study the role of metals in the production of biologics.



Chris Heger, PhD
Director of Applications Science,

Modernize Your Gene Therapy Analytics with
Automated Tools from Bio-Techne

December 7, 2021, at 11 am ET (8 am PT | 5 pm CET)

In this webinar, hear how a variety of innovative analytical tools can support your gene therapy workflow. Learn how automated systems can streamline your Western blotting workflow, characterize capsid proteins by size (MW) or charge (pI) based techniques in complex sample types, and identify contaminant species with high sensitivity. See how a combination of automated systems can also precisely characterize samples by size (MW) or charge (pI) using direct detection methods, assess particle contaminants in formulations, and improve your ELISA-based protein detection methods.




Explore our archive of on-demand webcasts