In this webinar, we will discuss the optimization of a capture step for the purification of a 5.3 kbp pDNA using Thermo Scientific^TM POROS^TM AEX resins. The Design of Experiments (DoE) approach, using small high-throughput screening columns, was completed to determine the optimal conditions for three different anion exchange chromatography resins with different surface chemistries. The greatest dynamic binding capacity was observed for a weak ion exchange resin (15 g/L) on 1-mL columns and these optimized conditions were confirmed using a scaled-down model. The advantages of POROS^TM resins in terms of pDNA binding capacity and impurity removal will be discussed to illustrate the capabilities of these chromatography media to facilitate the development of a large-scale plasmid purification process that can meet the growing demands of gene therapy and vaccine therapeutics.

In this webinar, hear how a variety of innovative analytical tools can support your gene therapy workflow. Learn how automated systems can streamline your Western blotting workflow, characterize capsid proteins by size (MW) or charge (pI) based techniques in complex sample types, and identify contaminant species with high sensitivity. See how a combination of automated systems can also precisely characterize samples by size (MW) or charge (pI) using direct detection methods, assess particle contaminants in formulations, and improve your ELISA-based protein detection methods.