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Culture of 3D Cell Aggregates in Perfusion |
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Research example with the Eppendorf DASbox® Mini Bioreactor System |
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Dear Reader,
Three-dimensional (3D) cell aggregates are of great interest for many applications, including disease modeling, drug toxicity assessment, and manufacture of stem cell-based products. Stirred-tank bioreactors are promising culture systems for 3D cell aggregates, as they allow efficient establishment and maintenance of cell aggregates, process monitoring and control, and process scale-up to larger volumes. Furthermore, they can be operated in perfusion mode, which allows 3D cell aggregates to be sustained longer than in traditional batch cultures. |
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Researchers at the Instituto de Biologia Experimental e Tecnológica (iBET) in Portugal have tested the suitability of the Eppendorf DASbox Mini Bioreactor System for the cultivation of the human tumor cell line H157 as 3D cell aggregates.
Download the application note and learn about the challenges and benefits of perfusion cultivation for stem cells and 3D cell models.
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