About this event:

Date: November 18, 2025

Time: 11 am EST | 8 am PST |
5 pm CET

Duration: 15 minutes

Sodium dodecyl sulfate capillary electrophoresis (CE-SDS) and capillary isoelectric focusing (cIEF) are two primary biochemical assays that provide information on purity and charge distribution of protein therapeutics. Subunit digests of therapeutic antibodies which cut above and below the hinge can provide further insight into product purity and location of PTMs.

This webinar presents a strategy for identification and localization of post translational modifications by analyzing subunit digests alongside native samples by CE-SDS. The workflow developed in this study may provide more insight into the localization of PTMs and the overall purity of a molecule at earlier stages of the drug development pipeline.

Key Takeaways: 

• Enhance detection of purity issues and PTMs in protein therapeutics with CE-SDS and icIEF-UV/MS.
• Analyze subunit digests with native samples help identify unknown antibody variants.
• Enable earlier, more accurate PTM and purity assessment in drug development.