--- title: Assessing Monomer and High Molecular Weight Species in Monoclonal Antibodies Using SE-HPLC description: In this webinar, learn best practices for assessing mAb monomer and HMW species using the validated SE-HPLC method. --- [![BPI Media_Logo 2_Color 1](https://bpi.bioprocessintl.com/hs-fs/hubfs/BPI%20Media_Logo%202_Color%201.png?width=350&height=161&name=BPI%20Media_Logo%202_Color%201.png)](https://bioprocessintl.com/) #                                          ![BPI Media_AsktheExpert_590 x 197_Header_for_Landing_Pages](https://bpi.bioprocessintl.com/hs-fs/hubfs/BPI%20Media_AsktheExpert_590%20x%20197_Header_for_Landing_Pages.jpg?width=543&height=181&name=BPI%20Media_AsktheExpert_590%20x%20197_Header_for_Landing_Pages.jpg) # Assessing Monomer and High Molecular Weight # Species in Monoclonal Antibodies Using SE-HPLC | | | --- | ## Presenters: ****![DerrickZhang](https://bpi.bioprocessintl.com/hs-fs/hubfs/DerrickZhang.png?width=160&height=230&name=DerrickZhang.png)**** **Derrick Zhang** *Senior Scientist II,* United States Pharmacopeia (USP)  [LinkedIn](https://www.linkedin.com/in/derrick-zhang-501708192/)   Derrick Zhang is a senior scientist II at the United States Pharmacopeia (USP), where he leads the development of strategies and standards supporting the analytical evaluation of monoclonal antibodies (mAbs) and therapeutic proteins, with deep expertise in Host Cell Protein (HCP) analysis and LC-MS proteomics. He brings over a decade of biopharmaceutical experience spanning process development, analytical characterization, and method development across organizations including Adaptive Phage Therapeutics, United Therapeutics, and Meso Scale Diagnostics.   ****![05-28-2026-USP-Seppallan-HS-Cropped](https://bpi.bioprocessintl.com/hs-fs/hubfs/05-28-2026-USP-Seppallan-HS-Cropped.png?width=160&height=232&name=05-28-2026-USP-Seppallan-HS-Cropped.png)**** **Shankar Sellappan, PhD** *Senior Product Manager* United States Pharmacopeia (USP)  [LinkedIn](https://www.linkedin.com/in/shankarsellappanphd/)     Shankar Sellappan, PhD is a senior product manager with the biologics group at USP, with more than 20 years of experience across biotechnology marketing and commercialization. His background spans life science research tools, mass spectrometry–based clinical diagnostics, and biopharmaceutical manufacturing and quality control.   A scientist by training, Shankar earned his doctoral degree in cancer biology from The University of Texas MD Anderson Cancer Center. He is passionate about demystifying complex scientific topics and translating them into clear, compelling messages that educate, engage, and help the broadest possible audience.   ## About this event: #### Date: June 9, 2026 #### Time: 11 am EDT | 8 am PDT | 5 pm CEST #### Duration: 60 minutes | Size exclusion high performance liquid chromatography (SE HPLC) is a cornerstone analytical technique for monitoring monoclonal antibody (mAb) product quality, enabling the measurement of monomer content and the detection of high molecular weight (HMW) species that may impact safety, efficacy, and stability. However, reliable interpretation of SE HPLC data depends on the use of validated methods and appropriate system suitability tools. In this webinar, USP will discuss best practices for assessing mAb monomer and HMW species using the validated SE-HPLC method described in USP–NF General Chapter ⟨129⟩, Analytical Procedures for Recombinant Therapeutic Monoclonal Antibodies. The session will highlight the role of the USP Monoclonal IgG System Suitability Reference Standard in establishing chromatographic suitability and helping ensure consistent method performance. In addition, USP will introduce the new USP High Molecular Weight Monoclonal IgG Reference Standard, designed to support column performance assessment prior to first use and throughout the operational lifetime of the column. Through practical examples, attendees will learn how compendial methods and reference standards can be used together to monitor aggregation, evaluate column health, and support robust, lifecycle focused control strategies for monoclonal antibody products. | | --- |   -   -   ©2015 BioProcess International All Rights reserved