Commercial Scale Insect Cell Culture: Opportunity and Challenges in a CHO Centric World

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Presenter:

Sharyn Farnsworth

Principal Scientist and Upstream Process Development-Cell Culture Group Leader

FUJIFILM Diosynth Biotechnologies

Sharyn Farnsworth is a Principal Scientist and group leader for the Upstream Process Development-Cell Culture team at FUJIFILM Diosynth Biotechnologies. She has over 22 years of experience in the process development and scale up of multiple cell lines including CHO, Sp2/0, NS0, HEK, SF9, and SF21 from 2-L to 10000-L volumes. Sharyn is the subject matter expert (SME) for Insect Cell Culture processes and single-use manufacturing at FUJIFILM Diosynth Biotechnologies including development activities and transfer in and scale up into good manufacturing practice (GMP) manufacturing. She successfully completed the implementation of single-use technologies into manufacturing driving the expansion of the company and played an integral role in the characterization of these technologies through kLa studies at all scales.

About this event:

Date: October 7, 2021

Time: 11 am EDT | 8 am PDT |
5 pm CEST

Duration: 15 minutes

A strategy for scale up and manufacture of a recombinant protein from Insect Cell Culture (ICC) using a Baculovirus Expression Vector System (BEVS) should be mapped out during the earliest stages of process development. The challenges of recombinant protein expression using ICC/BEVS production systems are different from more commonly used expression systems such as Chinese hamster ovary (CHO) and few vendors have the necessary scale-up experience to perform these assessments. The challenges include overcoming the inherent inconsistency of production media used for ICC, defining the appropriate cell cycling strategy, and, most importantly, determining the defining viral infection strategy. Keeping a line of sight to manufacturing helps to define the manufacturing process and ensure it is reliable and robust.