%PDF-1.3 1 0 obj << /Type /Catalog /Outlines 2 0 R /Pages 3 0 R >> endobj 2 0 obj << /Type /Outlines /Count 0 >> endobj 3 0 obj << /Type /Pages /Kids [6 0 R ] /Count 1 /Resources << /ProcSet 4 0 R /Font << /F1 8 0 R /F2 9 0 R /F3 10 0 R /F4 11 0 R >> /XObject << /I1 12 0 R /I2 13 0 R /I3 18 0 R /I4 19 0 R /I5 32 0 R /I6 33 0 R >> >> /MediaBox [0.000 0.000 595.280 841.890] >> endobj 4 0 obj [/PDF /Text /ImageC ] endobj 5 0 obj << /Creator (DOMPDF) /CreationDate (D:20220609140723-04'00') /ModDate (D:20220609140723-04'00') /Title (Continuous Culture of CB.Hep-1 Hybridoma Cell In Fifty Days Using A Bioreactor With A Stirring System Simulating Fish-Tails) >> endobj 6 0 obj << /Type /Page /Parent 3 0 R /Annots [ 14 0 R 16 0 R 20 0 R 22 0 R 24 0 R 26 0 R 28 0 R 30 0 R ] /Contents 7 0 R >> endobj 7 0 obj << /Length 3081 >> stream 1.000 1.000 1.000 rg 34.016 34.016 527.249 773.859 re f q 441.000 0 0 97.500 45.016 710.374 cm /I2 Do Q 0.000 0.000 0.000 rg BT 45.016 699.385 Td /F1 9.0 Tf [(Published on )] TJ ET BT 99.547 699.385 Td /F2 9.0 Tf [(IVT Network)] TJ ET BT 149.056 699.385 Td /F1 9.0 Tf [( \()] TJ ET BT 154.555 699.385 Td /F1 9.0 Tf [(http://www.ivtnetwork.com)] TJ ET 0.000 0.000 0.000 RG 0.18 w 0 J [ ] 0 d 154.555 698.234 m 259.081 698.234 l S BT 259.081 699.385 Td /F1 9.0 Tf [(\))] TJ ET 0.620 0.620 0.620 rg 45.016 686.888 506.159 0.750 re f 0.000 0.000 0.000 rg BT 45.016 662.142 Td /F3 15.0 Tf [(Continuous Culture of CB.Hep-1 Hybridoma Cell In Fifty Days Using A )] TJ ET BT 45.016 644.329 Td /F3 15.0 Tf [(Bioreactor With A Stirring System Simulating Fish-Tails)] TJ ET q 45.016 534.762 506.159 91.459 re W n 0.800 0.800 0.800 rg 45.016 625.845 15.512 0.750 re f 0.000 0.000 0.000 rg BT 45.016 576.370 Td /F1 9.0 Tf [(By)] TJ ET 0.800 0.800 0.800 rg 60.528 625.845 427.661 0.750 re f q 31.500 0 0 31.500 64.278 594.720 cm /I4 Do Q 0.000 0.000 0.000 rg BT 488.189 615.231 Td /F4 9.0 Tf [(Hasel Aragn, Marcos Gonzlez, Grechen Menndez, Daily Hernndez, Williams Ferro, David )] TJ ET BT 488.189 601.495 Td /F4 9.0 Tf [(Goitizolo, Tatiana lvarez, Yanet Villegas, Jos Garca, Tatiana Gonzlez, Regla Margarita, Maiyln )] TJ ET BT 488.189 587.759 Td /F4 9.0 Tf [(LaO, Andrs Tamayo, Rodolfo Valds)] TJ ET 0.800 0.800 0.800 rg 488.189 625.845 62.985 0.750 re f 0.000 0.000 0.000 rg BT 488.189 585.113 Td /F4 9.0 Tf [(May 27, 2020 )] TJ ET BT 488.189 571.377 Td /F4 9.0 Tf [(8:08 am EDT)] TJ ET Q BT 45.016 395.523 Td /F1 9.0 Tf [(ABSTRACT Since mammalian cells were isolated and cultivated under controlled conditions; several bioreactor types, )] TJ ET BT 45.016 381.786 Td /F1 9.0 Tf [(operation modes and culture media have been assessed to obtain products with the highest purity and yield. In such sense, )] TJ ET BT 45.016 368.050 Td /F1 9.0 Tf [(the Lambda-MINIFOR bioreactor uses an unusual vertical stirring system for maximizing the mixture of gases and nutrients )] TJ ET BT 45.016 354.314 Td /F1 9.0 Tf [(and reducing cellular damages. Therefore, the paper subject was to study continuous culture of CB.Hep-1 hybridoma cell in )] TJ ET BT 45.016 340.578 Td /F1 9.0 Tf [(protein free medium using Lambda-MINIFOR bioreactor in fifty days. Results revealed 1.49 0.27 x 106 cell/mL as average...)] TJ ET q 45.016 326.580 506.159 0.000 re W n Q 0.620 0.620 0.620 rg 45.016 285.330 506.159 0.750 re f 0.000 0.000 0.000 rg BT 45.016 267.805 Td /F4 10.7 Tf [(Source URL:)] TJ ET BT 112.587 267.805 Td /F1 10.7 Tf [(http://www.ivtnetwork.com/article/continuous-culture-cbhep-1-hybridoma-cell-fifty-days-)] TJ ET 0.000 0.000 0.000 RG 0.21336 w 0 J [ ] 0 d 112.587 266.440 m 522.814 266.440 l S BT 45.016 251.523 Td /F1 10.7 Tf [(using-bioreactor-stirring-system-simula)] TJ ET 0.21336 w 0 J [ ] 0 d 45.016 250.158 m 228.761 250.158 l S 0.890 0.929 0.737 RG 1.5 w 0 J [ ] 0 d 64.516 414.762 147.750 111.750 re S q 146.250 0 0 110.250 65.266 415.512 cm /I6 Do Q endstream endobj 8 0 obj << /Type /Font /Subtype /Type1 /Name /F1 /BaseFont /Helvetica /Encoding /WinAnsiEncoding >> endobj 9 0 obj << /Type /Font /Subtype /Type1 /Name /F2 /BaseFont /Helvetica-Oblique /Encoding /WinAnsiEncoding >> endobj 10 0 obj << /Type /Font /Subtype /Type1 /Name /F3 /BaseFont /Times-Roman /Encoding /WinAnsiEncoding >> endobj 11 0 obj << /Type /Font /Subtype /Type1 /Name /F4 /BaseFont /Helvetica-Bold /Encoding /WinAnsiEncoding >> endobj 12 0 obj << /Type /XObject /Subtype /Image /Width 588 /Height 130 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 588 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 8715>> stream x]OK#MDA+x !\K ބ {o/|ygz&Y}Nt]]]UyD~|VVd`蓨CFAgy~u2W!Bm}u%2gSFDK_]惂la蓨-L}U)ϢKݯDFsA0_]恊W"+ Sk<`k
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