%PDF-1.3 1 0 obj << /Type /Catalog /Outlines 2 0 R /Pages 3 0 R >> endobj 2 0 obj << /Type /Outlines /Count 0 >> endobj 3 0 obj << /Type /Pages /Kids [6 0 R 25 0 R 27 0 R 29 0 R 32 0 R 35 0 R 37 0 R 39 0 R 41 0 R 43 0 R 47 0 R 53 0 R 56 0 R 59 0 R 62 0 R 65 0 R ] /Count 16 /Resources << /ProcSet 4 0 R /Font << /F1 8 0 R /F2 9 0 R /F3 10 0 R /F4 11 0 R /F5 12 0 R /F6 49 0 R >> /XObject << /I1 13 0 R /I2 14 0 R /I3 23 0 R /I4 24 0 R /I5 31 0 R /I6 34 0 R /I7 52 0 R /I8 55 0 R /I9 58 0 R /I10 61 0 R /I11 64 0 R /I12 67 0 R /I13 68 0 R /I14 69 0 R >> >> /MediaBox [0.000 0.000 595.280 841.890] >> endobj 4 0 obj [/PDF /Text /ImageC ] endobj 5 0 obj << /Creator (DOMPDF) /CreationDate (D:20220607114618-04'00') /ModDate (D:20220607114618-04'00') /Title (Impact Of Ligand Density On Hepatitis B Virus Surface Antigen Immunoaffinity Chromatography Efficiency And Ligand Leakage) >> endobj 6 0 obj << /Type /Page /Parent 3 0 R /Annots [ 15 0 R 17 0 R 19 0 R 21 0 R ] /Contents 7 0 R >> endobj 7 0 obj << /Length 4698 >> stream 1.000 1.000 1.000 rg 34.016 34.016 527.249 773.859 re f q 441.000 0 0 97.500 45.016 710.374 cm /I2 Do Q 0.000 0.000 0.000 rg BT 45.016 699.385 Td /F1 9.0 Tf [(Published on )] TJ ET BT 99.547 699.385 Td /F2 9.0 Tf [(IVT Network)] TJ ET BT 149.056 699.385 Td /F1 9.0 Tf [( \()] TJ ET BT 154.555 699.385 Td /F1 9.0 Tf [(http://www.ivtnetwork.com)] TJ ET 0.000 0.000 0.000 RG 0.18 w 0 J [ ] 0 d 154.555 698.234 m 259.081 698.234 l S BT 259.081 699.385 Td /F1 9.0 Tf [(\))] TJ ET 0.620 0.620 0.620 rg 45.016 686.888 506.159 0.750 re f 0.000 0.000 0.000 rg BT 45.016 662.142 Td /F3 15.0 Tf [(Impact Of Ligand Density On Hepatitis B Virus Surface Antigen Immunoaffinity )] TJ ET BT 45.016 644.329 Td /F3 15.0 Tf [(Chromatography Efficiency And Ligand Leakage)] TJ ET q 45.016 579.998 506.159 46.223 re W n 0.800 0.800 0.800 rg 45.016 625.845 18.579 0.750 re f 0.000 0.000 0.000 rg BT 45.016 598.988 Td /F1 9.0 Tf [(By)] TJ ET 0.800 0.800 0.800 rg 63.595 625.845 399.218 0.750 re f 0.000 0.000 0.000 rg BT 63.595 607.731 Td /F4 9.0 Tf [(Mayte Quintana, Williams Ferro, Airela Llamo, Tatiana Gonzlez, Miguel Castillo, Yenisley )] TJ ET BT 63.595 593.995 Td /F4 9.0 Tf [(Medina, Yanet Villegas, and Rodolfo Valds)] TJ ET 0.800 0.800 0.800 rg 462.813 625.845 88.361 0.750 re f 0.000 0.000 0.000 rg BT 462.813 607.731 Td /F4 9.0 Tf [(Jun 25, 2020 7:00 )] TJ ET BT 462.813 593.995 Td /F4 9.0 Tf [(am EDT)] TJ ET Q BT 45.016 364.401 Td /F4 10.5 Tf [(ABSTRACT)] TJ ET BT 45.016 339.668 Td /F1 9.0 Tf [(Hepatitis B surface antigen \(HBsAg\) can be purified by immunoaffinity chromatography for human vaccination, which involves )] TJ ET BT 45.016 325.931 Td /F1 9.0 Tf [(key aspects such as support \(Agarose\) and ligand \(monoclonal antibodies \(mAb\)\). Theoretically, an increase in )] TJ ET BT 45.016 312.195 Td /F1 9.0 Tf [(immunosorbent ligand density could provoke a decrease in elution capacity, elution efficiency and recovery of )] TJ ET BT 45.016 298.459 Td /F1 9.0 Tf [(immunosorbents. To corroborate this hypothesis, the impact of five CB.Hep-1 mAb ligand densities \(2.67 - 3.78 mg/mL\) on )] TJ ET BT 45.016 284.723 Td /F1 9.0 Tf [(HBsAg immunopurification capacity and ligand leakage was analyzed in 23 purification cycles applying purified HBsAg )] TJ ET BT 45.016 270.986 Td /F1 9.0 Tf [(samples.)] TJ ET BT 45.016 246.720 Td /F1 9.0 Tf [(Next, ligand density that revealed better purification results applying purified HBsAg samples was challenged with HBsAg )] TJ ET BT 45.016 232.984 Td /F1 9.0 Tf [(unpurified samples in five purification cycles. As results, 3.15 mg/mL-CB.Hep-1 mAb-immunosorbent showed 105.1 16 g )] TJ ET BT 45.016 219.248 Td /F1 9.0 Tf [(HBsAg/mg mAb as adsorption capacity, 91.5 10.4 g HBsAg/mg mAb as elution capacity, 58.9 6.6 % as HBsAg recovery, )] TJ ET BT 45.016 205.511 Td /F1 9.0 Tf [(69.5 10.3 % as adsorption efficiency, 84.8 6.8 % as elution efficiency and 0.57 0.43 ng mAb/g HBsAg as ligand )] TJ ET BT 45.016 191.775 Td /F1 9.0 Tf [(leakage. The challenge of 3.15 mg/mL-CB.Hep-1 mAb-immunosorbent with HBsAg unpurified samples also evidenced )] TJ ET BT 45.016 178.039 Td /F1 9.0 Tf [(significant differences \(p< 0.005\) in adsorption capacity, elution capacity, elution efficiency, recovery and mAb released from )] TJ ET BT 45.016 164.303 Td /F1 9.0 Tf [(immunosorbent As conclusion, the ligand density increase has a significant negative impact on immunosorbent elution )] TJ ET BT 45.016 150.566 Td /F1 9.0 Tf [(efficiency for HBsAg purification and 3.15 mg/mL is the most suitable ligand density for immunosorbent based on Zetarose )] TJ ET BT 45.016 136.830 Td /F1 9.0 Tf [(CL4B to purify HBsAg allowing twenty-three purification cycles, a recovery higher than fifty percent, a ligand leakage lower )] TJ ET BT 45.016 123.094 Td /F1 9.0 Tf [(than approved limit and a notable reduction in immunosorbent production process cost.)] TJ ET BT 45.016 96.959 Td /F4 10.5 Tf [(INTRODUCTION)] TJ ET BT 45.016 72.226 Td /F1 9.0 Tf [(According to the World Health Organization, there are more than 350 million Hepatitis B virus \(HBV\) chronic carriers )] TJ ET 0.890 0.929 0.737 RG 1.5 w 0 J [ ] 0 d 64.516 461.498 147.750 110.250 re S q 146.250 0 0 108.750 65.266 462.248 cm /I4 Do Q 0.776 0.776 0.776 rg 0.776 0.776 0.776 RG 63.766 395.288 m 212.266 395.288 l 212.266 396.038 l 63.766 396.038 l f 0.439 0.439 0.439 rg BT 63.766 442.556 Td /F5 9.0 Tf [(Stability in twenty-three purification )] TJ ET BT 63.766 429.191 Td /F5 9.0 Tf [(cycles of the CB.Hep-1 mAb )] TJ ET BT 63.766 415.826 Td /F5 9.0 Tf [(immunosorbents applying HBsAg )] TJ ET BT 63.766 402.461 Td /F5 9.0 Tf [(purified samples)] TJ ET endstream endobj 8 0 obj << /Type /Font /Subtype /Type1 /Name /F1 /BaseFont /Helvetica /Encoding /WinAnsiEncoding >> endobj 9 0 obj << /Type /Font /Subtype /Type1 /Name /F2 /BaseFont /Helvetica-Oblique /Encoding /WinAnsiEncoding >> endobj 10 0 obj << /Type /Font /Subtype /Type1 /Name /F3 /BaseFont /Times-Roman /Encoding /WinAnsiEncoding >> endobj 11 0 obj << /Type /Font /Subtype /Type1 /Name /F4 /BaseFont /Helvetica-Bold /Encoding /WinAnsiEncoding >> endobj 12 0 obj << /Type /Font /Subtype /Type1 /Name /F5 /BaseFont /Times-Bold /Encoding /WinAnsiEncoding >> endobj 13 0 obj << /Type /XObject /Subtype /Image /Width 588 /Height 130 /Filter /FlateDecode /DecodeParms << /Predictor 15 /Colors 1 /Columns 588 /BitsPerComponent 8>> /ColorSpace /DeviceGray /BitsPerComponent 8 /Length 8715>> stream x]OK#MDA+x !\K ބ {o/|ygz&Y}Nt]]]UyD~|VVd`蓨CFAgy~u2W!Bm}u%2gSFDK_]惂la蓨-L}U)ϢKݯDFsA0_]恊W"+ Sk<`k
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