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title: Integrated and Intensified Downstream Processing Within a Continuous Multi-Column Chromatography Platform
description: Multi-column chromatography integrates a one system mAb capture and polish with inline virus inactivation for continuous, sustainable downstream processing.
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# Integrated and Intensified Downstream

# Processing Within a Continuous Multi-Column

# Chromatography Platform

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## ![Picture2](https://bpi.bioprocessintl.com/hs-fs/hubfs/Picture2.png?width=300&height=111&name=Picture2.png)

## Presenter:

****![09-08-2026-Tosoh-ATE-Speaker-HS-Cropped](https://bpi.bioprocessintl.com/hs-fs/hubfs/09-08-2026-Tosoh-ATE-Speaker-HS-Cropped.jpg?width=160&height=232&name=09-08-2026-Tosoh-ATE-Speaker-HS-Cropped.jpg)****

**Abbie Breton**

*Applications Development Manager,*

Tosoh Bioscience  

 

Abbie Breton is the manager of application development and acting applications scientist for multi-column chromatography systems at Tosoh Bioscience. Abbie joined Tosoh in 2019, bringing with her experience from her early career executing viral clearance studies for a CRO. In her current role, Abbie supports the development and application of advanced chromatography solutions, working closely with internal teams and customers to advance bioprocessing technologies.

## About this event:

#### Date: September 8, 2026

#### Time: 11 am EDT | 8 am PDT | 5 pm CEST

#### Duration: 30 minutes

| Achieving true continuity in downstream processing remains a key challenge for monoclonal antibody (mAb) production. Here, multi-column chromatography (MCC) is advanced beyond continuous capture to enable direct integration of a subsequent chromatographic purification step on a single system. Previous integration of downstream steps has been constrained by equipment and process limitations. The adapted multi-column system used in this work integrates two chromatographic steps in a single eight-column setup, supporting uninterrupted operation and efficient resin use. Using a high alkaline stable, fast kinetic ligand, and high dynamic binding capacity (DBC) Protein A affinity resin, Tosoh demonstrates robust mAb capture and subsequent polishing with a highly salt-tolerant cation exchange resin, advancing practical implementation of end-to-end continuous purification. In addition, a low-threshold pH step was applied into this process integration to achieve inline virus inactivation. A high degree of automation has been achieved with this setup, while the footprint has been reduced both by reducing the size of the systems and by saving on cost of buffers, thereby making downstream processing more sustainable.  Key Takeaways:  - Understand how MCC can streamline mAb DSP. - Optimize a mAb purification process using two highly efficient chromatography resins. - Learn how MCC plays a pivotal role in continuous processing. |
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