Reliable column packing is fundamental to reproducible chromatography, but the optimal approach depends on resin properties, chromatographic mode, column dimensions, and scale.

This webcast will compare practical packing considerations across different chromatography resin chemistries and bead types, highlighting how these characteristics can influence slurry preparation, packing flow, pressure, compression, and final bed quality. Particular attention will be given to differences between ion exchange (IEX) and hydrophobic interaction chromatography (HIC) media, as well as the considerations that arise when transferring packing methods from small laboratory columns to larger process-scale columns.

The presentation will also cover slurry concentration determination and why accurate slurry concentration is an important starting point for achieving the intended bed volume and reproducible column packing.

Key Takeaways:

• Understand why different resin chemistries and bead types may require different packing strategies.

• Recognize practical differences when packing IEX and HIC media.

• Learn approaches for determining and adjusting slurry concentration before packing.

• Identify packing parameters that should be reconsidered when moving from laboratory- to process-scale columns.

• Use pressure-flow behavior and chromatographic performance as practical tools for evaluating packing quality.