---
title: Purification of DNA Oligonucleotides Using Anion Exchange Chromatography
description: In this webcast, see data from the Nuvia HP-Q Resin, a high-performance strong anion exchanger, used for the purification of two oligonucleotides having a phosphodiester backbone.
---

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# Purification of DNA Oligonucleotides Using

# Anion Exchange Chromatography

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## Presenter:

****![eric savillex 05-25 headshot](https://bpi.bioprocessintl.com/hs-fs/hubfs/8-4-22-BioRad-ATE-Rushton.png?width=168&name=8-4-22-BioRad-ATE-Rushton.png)****

**William H. Rushton**

*Process Chromatography Support Scientist,*

Bio-Rad Laboratories

 

William H. Rushton is a Chromatography Support Scientist for Bio-Rad. Prior to Bio-Rad, he worked at Centocor from 1997-2007 in the Process Development Group. He developed the downstream purification processes for Simponi® and Stelara®. He joined Charles River Laboratories in 2007 as Manager of the Process Validation Group performing viral clearance studies. In 2009, he moved to Auxilium Pharmaceuticals as a senior scientist in process development. He supported the BLA filing and post marketing activities for Xiaflex®. He obtained a MS from Philadelphia College of Osteopathic Medicine and BS from St. Joseph’s University.

## About this event:

#### Date: August 4, 2022

#### Time: 11 am EDT | 8 am PDT | 5 pm CET

#### Duration: 15 minutes

DNA oligonucleotides obtained by solid-phase synthesis contain incomplete or erroneous sequences that require removal for the advancement of these molecules as therapeutics. These key impurities in crude samples are length-based and are best removed by anion exchange chromatography.

 

In this webcast, data will be presented using Nuvia HP-Q Resin, a high-performance strong anion exchanger, used for the purification of two oligonucleotides (20-mer and 21-mer) having a phosphodiester backbone. Both yield (~90%) and purity (~98%) data were obtained during scale-up studies and will be discussed. In addition, the data indicates that this strong anion exchange resin can also be used for the efficient purification of fully thiolated oligonucleotides used in clinical trials.

Key Takeaways:

- Impurities are best removed by strong anion exchange chromatography.
- Key process parameters were optimized to increase yield and purity.
- A cost-effective, efficient, and scalable purification method was achieved.

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