Stem Cell Derived Exosome Production in Stirred-Tank Bioreactors

Stem Cell Derived Exosome Production
in Stirred-Tank Bioreactors

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Presenters:

Aurélie Tacheny

Project Manager and Application Specialist
Eppendorf

After a PhD in cell biology obtained at the University of Namur, Belgium, and more than 10 years in the academic field, Aurélie Tacheny has worked for six years as a Project Manager and Application Specialist for Eppendorf Application Technologies. Responsible for various cell-culture–related projects, Aurélie and her team in the lab use their expertise in this field to generate valuable experimental data and support current and future Eppendorf products and customers.

Jorge Escobar
Senior Research Scientist, Applications Lab
Eppendorf

Jorge Escobar is a senior research scientist in the applications lab of Eppendorf, Inc., in Enfield, Connecticut. He joined the company in 2019 and has worked on applied research focused on efficient expansion of adherent and nonadherent cells (e.g., stem cells, adult primary cells) in cell culture bioreactors. After his PhD in biomedical engineering at the Polytechnic University of Valencia (UPV, Valencia, Spain), he gained experience in the fields of cell therapy and regenerative medicine working as a researcher at the Center for Biomaterials and Tissue Engineering (UPV), Institute of Biomaterial Sciences, Helmholtz-Zentrum Geesthacht (Berlin, Germany) and the UConn Health Institute for Regenerative Engineering (Farmington, Connecticut). In 2017, he was promoted to Assistant Research Professor, Department of Chemical and Biomolecular Engineering, University of Connecticut.

About this event:

Date: March 4, 2021

Time: 11 am EST | 8 am PST |
5 pm CET

Duration: 45 minutes

Exosomes are a population of naturally occurring, mobile, membrane-limited,
30–100 nm in diameter, extracellular vesicles containing a large number of proteins, lipids, messenger, and micro-RNAs. It was shown that they play a role in the mediation of intercellular communication, the modulation of immune-regulatory processes, tumor metabolism, and regenerative as well as degenerative processes. In recent years, there has been increasing interest in the therapeutic potential of exosomes produced by mesenchymal stem cells (MSCs).

Stirred-tank bioreactors offer the possibility to tightly control and monitor the production of exosomes, as well as the scalability, to produce increasing amounts. However, the cultivation of stem cells in stirred-tank bioreactors requires profound knowledge and precise control of the process.

In our talk, we will highlight the potential and benefits of stirred-tank bioreactors in the cultivation of stem cells for exosome production. We will share our knowledge on how we developed the process in our DASbox Mini Parallel Bioreactor System and scaled up the process to 1 L controlled by our newest parallel bioreactor control system, the SciVario twin.

Target groups:

Scientists new to the cultivation of stem cells in bioreactors
Experienced scientists looking for tips and tricks to improve their stem cell culture and EV production processes

Key learning objectives:

Learn how to cultivate stem cells in a stirred-tank bioreactor: how to identify the right cultivation method, vessel type, vessel material, and instrument configuration for your specific needs.
Learn how to define and optimize important parameters to improve culture results, including tips and tricks.
Deepen your knowledge and optimize your process through this live "Ask the Expert" session.